ANALYTICAL TECHNIQUES FOR CELL-FRACTIONS .26. TWO-DIMENSIONAL ELECTROPHORETIC ANALYSIS OF BASIC-PROTEINS USING PHOSPHATIDYL CHOLINE UREA SOLUBILIZATION

被引:67
作者
WILLARD, KE
GIOMETTI, CS
ANDERSON, NL
OCONNOR, TE
ANDERSON, NG
机构
[1] Molecular Anatomy Program, Division of Biological and Medical Research, Argonne National Laboratory, Argonne
关键词
D O I
10.1016/0003-2697(79)90232-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The ISO-DALT system of two-dimensional electrophoresis allows high-resolution separations of proteins and protein subunits. However, the conventional isoelectric focusing employed in this system does not give satisfactory resolution of the more basic proteins, such as histones. The BASO-DALT system was designed to obtain improved resolution of these basic proteins in the first dimension. In this system, phosphatidyl choline is used as the solubilization agent, and allows resolution of many low molecular weight basic proteins that were not seem with more conventional detergents. Using the BASO-DALT system, Novikoff hepatoma chromosomal proteins have been analyzed, and the five histones identified. © 1979.
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页码:289 / 298
页数:10
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