CA2+ REGULATED MODULATOR PROTEIN INTERACTING AGENTS - INHIBITION OF CA2+-MG2+-ATPASE OF HUMAN-ERYTHROCYTE GHOST

被引：82

作者：

KOBAYASHI, R ^{[1
]}

TAWATA, M ^{[1
]}

HIDAKA, H ^{[1
]}

机构：

[1] SHINSHU UNIV,SCH MED,INST ADAPTAT MED,DEPT MED,MATSUMOTO 390,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 88卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91513-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Agents such as N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), and its derivatives, chlorpromazine and amitriptyline that interact with calcium-regulated modulator protein were found to inhibit not only Ca2+ dependent cyclic nucleotide phosphodiesterase but also Ca2+-Mg2+-ATPase of human erythrocyte ghosts. I50 values of modulator interacting agents for testis modulator-activated, brain modulator-activated and erythrocyte modulator-activated-ATPase are indistinguishable. However, I50 of W-7 for troponin C-activated-ATPase is lower than that for modulator-activated ATPase. The specificity of these agents toward modulator-related enzyme reaction is also shown by the negative effect on modulator-unrelated enzyme system such as erythrocyte ghost protein kinase and Mg2+-ATPase. These agents provide a useful tool for elucidating the physiological role of modulator. © 1979.

引用

页码：1037 / 1045

页数：9

共 20 条

[1] SOLUBLE-PROTEIN ACTIVATOR OF (MG2++CA2+)-DEPENDENT ATPASE IN HUMAN RED-CELL MEMBRANES [J].

BOND, GH ;

CLOUGH, DL .

BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 323 (04) :592-599

[2] CYCLIC 3,5-NUCLEOTIDE PHOSPHODIESTERASE . DEMONSTRATION OF AN ACTIVATOR [J].

CHEUNG, WY .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1970, 38 (03) :533-&

[3] TROPONIN AND ITS COMPONENTS [J].

EBASHI, S ;

WAKABAYASHI, T ;

EBASHI, F .

JOURNAL OF BIOCHEMISTRY, 1971, 69 (02) :441-+

[4]

Fiske CH, 1925, J BIOL CHEM, V66, P375

[5] PHOSPHODIESTERASE PROTEIN ACTIVATOR MIMICS RED BLOOD-CELL CYTOPLASMIC ACTIVATOR OF (CA2+-MG2+)ATPASE [J].

GOPINATH, RM ;

VINCENZI, FF .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1977, 77 (04) :1203-1209

[6]

HIDAKA H, 1978, BLOOD VESSELS, V15, P55

[7]

HIDAKA H, 1978, J PHARMACOL EXP THER, V207, P8

[8]

HIDAKA H, 1979, MOL PHARMACOL, V15, P49

[9]

HONDA F, 1973, JAP J PHARMACOL S27, V23

[10] ANALYSIS OF AUTOPHOSPHORYLATION OF RABBIT AND HUMAN ERYTHROCYTE-MEMBRANES [J].

HOSEY, MM ;

TAO, M .

BIOCHEMISTRY, 1976, 15 (07) :1561-1568

← 1 2 →