A TURBIDIMETRIC ASSAY IN AN ELISA READER FOR THE DETERMINATION OF MONONUCLEAR PHAGOCYTE PROCOAGULANT ACTIVITY

被引：27

作者：

JUNGI, TW

机构：

[1] Institute of Veterinary Virology, University of Berne, Berne

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 133卷 / 01期

关键词：

Coagulation; Endotoxin; Macrophage; Methodology; Monocyte; Procoagulant activity; Thromboplastin;

D O I：

10.1016/0022-1759(90)90314-L

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for the simultaneous determination of plasma recalcification time in 60-96 replicates. It is based on turbidimetry in a thermostatic (37°C) ELISA reader photometer. Dose-response curves with thromboplastin showed a double-logarithmic correlation between recalcification time and thromboplastin concentration. Various ways of determining the coagulation time by turbidimetry were compared. Samples of mononuclear cells or monocyte-derived macrophages showed that procoagulant activity (PCA), expressed in thromboplastin units, was directly proportional to the cell number. The method may be of value for the determination of mononuclear phagocyte PCA and for other applications of plasma recalcification time. © 1990.

引用

页码：21 / 29

页数：9

共 28 条

[1] EXPERIMENTAL GRAM-NEGATIVE SEPTICEMIA - THROMBOPLASTIN GENERATION IN MONONUCLEAR PHAGOCYTES FROM DIFFERENT ANATOMICAL SITES [J].