A GENERAL-METHOD FOR THE PREPARATION OF MIXED MICELLES OF HYDROPHOBIC PEPTIDES AND SODIUM DODECYL-SULFATE

被引：50

作者：

KILLIAN, JA

TROUARD, TP

GREATHOUSE, DV

CHUPIN, V

LINDBLOM, G

机构：

[1] UMEA UNIV,DEPT PHYS CHEM,UMEA,SWEDEN

[2] UNIV ARKANSAS,DEPT CHEM & BIOCHEM,FAYETTEVILLE,AR 72701

来源：

FEBS LETTERS | 1994年 / 348卷 / 02期

关键词：

MICELLE; CIRCULAR DICHROISM; PEPTIDE STRUCTURE; SIGNAL PEPTIDE; GRAMICIDIN; 2-DIMENSIONAL NMR;

D O I：

10.1016/0014-5793(94)00594-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method is reported for the incorporation of hydrophobic peptides into sodium dodecyl sulphate (SDS) micelles. First, a homogeneous solution of peptide and detergent is obtained by adding the peptide in trifluoroethanol to an equal volume of an aqueous solution of SDS. Upon subsequent addition of excess water, mixed peptide-SDS micelles are formed. Next, all solvent is removed by lyophilization and an appropriate amount of water is added to the dry powder. For various hydrophobic peptides this was shown to yield clear and stable solutions that are highly concentrated and suitable for characterization by spectroscopic techniques.

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页码：161 / 165

页数：5

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