AMPEROMETRIC DETERMINATION OF DOPAMINE ON AN ENZYMATICALLY MODIFIED CARBON-PASTE ELECTRODE

被引：89

作者：

FORZANI, ES

RIVAS, GA

SOLIS, VM

机构：

[1] INFIQC, Departamento de Físico Química, Facultad de Ciencias Químicas, 5016 Córdoba, Sucursal 16

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1995年 / 382卷 / 1-2期

关键词：

AMPEROMETRY; ENZYME ELECTRODE; DOPAMINE; CARBON PASTE ELECTRODE;

D O I：

10.1016/0022-0728(94)03640-O

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

The amperometric determination of dopamine under controlled convective conditions on an enzymatically modified carbon paste electrode was studied using potato tissue as a source of polyphenol oxidase. Enzymatic kinetic constants and substrate diffusional-resistance parameters were determined and an analysis of the most common interferents was made. It is concluded that the electrode is suitable for the quantification of dopamine with a detection limit as low as 2.5 x 10(-6) M when the hydrodynamic conditions are carefully controlled. The electrode is active towards dopac, ascorbic acid and pyrocatechol but not L-tyrosine, L-dopa, homovanillic acid, adrenaline, normetanephrine and vanilmandelic acid.

引用

页码：33 / 40

页数：8

共 31 条

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