THE RAPIDLY PHOSPHORYLATED 25-KDA POLYPEPTIDE OF THE LIGHT-HARVESTING COMPLEX OF PHOTOSYSTEM-II IS ENCODED BY THE TYPE-2 CAB-II GENES

被引：33

作者：

JANSSON, S

SELSTAM, E

GUSTAFSSON, P

机构：

[1] Department of Plant Physiology, University of Umeå, Umeå

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1019卷 / 02期

关键词：

Acylation; Gene product; Light harvesting complex; Peripheral LHC II; Photosystem II; Type 2 cab-II gene;

D O I：

10.1016/0005-2728(90)90130-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The main light-harvesting complex of Photosystem II (LHC II) in higher plants consists of two sub-populations. The 'inner' pool consists only of a 27 kDa polypeptide, whereas in the 'outer' pool both the 27 kDa and a 25 kDa polypeptide are found. We purified the 25 and the 27 kDa LHC II polypeptides from Scots pine and 25 kDa LHC II polypeptide from spinach. Protein sequencing after cleavage with endoproteinase Lys-C showed that the 25 kDa polypeptide is encoded by the Type 2 cab-II genes and the 27 kDa polypeptide by the Type 1 cab-II genes. A fatty acid was not covalently attached to the peptides assembled into the pigment-protein complex. Our results show that the different polypeptides seen on a gel are different gene products, and not the result of different processing. © 1990.

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页码：110 / 114

页数：5

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