A NEW SENSITIVE FLUOROMETRIC ASSAY FOR THE METABOLISM OF (-)-7,8-DIHYDROXY-7,8-DIHYDROBENZO[A]PYRENE BY HUMAN HAIR-FOLLICLES

被引：14

作者：

ALEXANDROV, K

ROJAS, M

GOLDBERG, M

CAMUS, AM

BARTSCH, H

机构：

[1] Unit of Environmental Carcinogens and Host Factors, International Agency for Research on Cancer, 69372 Lyon Cedex 08

[2] CNRS, BP 2

来源：

CARCINOGENESIS | 1990年 / 11卷 / 12期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1093/carcin/11.12.2157

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A new sensitive fluorometric assay was established to measure the stereospecific formation of benzo[a]pyrene tetrols formed after cytochrome P450-dependent metabolism of (-)-7,8-dihydroxy-7,8-dihydrobenzo[a]pryene by human hair follicles. This simple assay requires three human hair follicles and a low (0.5-2.0 mu-M) substrate concentration and has a limit of detection of approximately 0.3 fmol of tetrols. Freshly isolated human hair follicles from 20 adult volunteers (10 non-smokers and 10 smokers) were assayed. While intersubject and seasonal variations were observed, the assay was found to be reproducible for a given subject. This rapid and non-invasive assay provides a new means for metabolic phenotyping of human subjects for their capacity to metabolize (-)-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene to its carcinogenic form (+)anti-benzo[a]pyrene diolepoxide.

引用

页码：2157 / 2161

页数：5

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