PRELIMINARY STUDIES ON THE CRYSTALLIZATION OF BEEF-HEART CYTOCHROME-C-OXIDASE BY VAPOR DIFFUSION

被引：8

作者：

ZAMUDIO, I ^{[1
]}

KORNBLATT, J ^{[1
]}

NICHOLLS, P ^{[1
]}

LI, Y ^{[1
]}

CYGLER, M ^{[1
]}

机构：

[1] CONCORDIA UNIV,DEPT BIOL,1455 MAISONNEUVE O,MONTREAL H3G 1M8,QUEBEC,CANADA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 169卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1016/0006-291X(90)92009-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Beef heart cytochrome c oxidase has been crystallized in its native and delipidated forms, using the hanging drop vapor diffusion method. The delipidated oxidase, which is subunit III deficient, can be crystallized in rosettes using this method from a medium containing zinc chloride as well as ammonium sulfate. The crystals give a protein 'powder' pattern on preliminary X-ray analysis. The crystals initially grow rapidly but stop growing at a small size probably as a result of addition of heterogeneous material at the crystal surface. © 1990.

引用

页码：1105 / 1110

页数：6

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