THE USE OF DIPHENYLENE IODONIUM AND ITS ANALOGS TO INVESTIGATE THE ROLE OF THE NADPH OXIDASE IN THE TUMORICIDAL ACTIVITY OF MACROPHAGES INVITRO

被引：23

作者：

HANCOCK, JT ^{[1
]}

WHITE, JI ^{[1
]}

JONES, OTG ^{[1
]}

SILVER, IA ^{[1
]}

机构：

[1] UNIV BRISTOL,SCH MED SCI,DEPT BIOCHEM,BRISTOL BS8 1TD,AVON,ENGLAND

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1991年 / 11卷 / 01期

关键词：

MACROPHAGES; OXYGEN RADICALS; TUMORICIDAL ACTIVITY; FREE RADICALS;

D O I：

10.1016/0891-5849(91)90184-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipopolysaccharide (LPS) was shown to induce tumoricidal activity in peritoneal macrophages. The optimal concentration was found to be 25-mu-g/mL. Approximately 20-h exposure to LPS was required before maximal tumor cell killing was attained. Optimal tumor killing was obtained with a ratio of tumor cell to macrophages of 1:40 with the macrophages in a confluent layer. Diphenylene iodonium (DPI) reduced the tumor cell killing in a dose dependent manner up to 1-mu-M. Under similar conditions DPI was shown to inhibit the superoxide production of macrophages and this supports the view that the production of oxygen radicals is important in the killing of tumor cells by macrophages and that the inhibitor DPI can be used to investigate their contribution to cytotoxicity.

引用

页码：25 / 29

页数：5

共 26 条

[1] Jones, Cross, Polymorphonuclear leukocytes in acute inflammation, Immunopathic mechanisms of arthritis, (1988)
[2] Ellis, Mayer, Jones, The effect of the NADPH oxidase inhibitor diphenylene iodonium on aerobic and anaerobic microbicidal activities of human neutrophils, Biochem. J., 251, pp. 887-891, (1988)
[3] Hancock, Jones, The inhibition by diphenylene iodonium and its analogues of superoxide generation by macrophages, Biochem. J., 242, pp. 103-107, (1987)
[4] Collette, McGreer, Crawford, Chubb, Sandin, Synthesis of some iodonium salts, J. Am. Chem. Soc., 78, pp. 3819-3820, (1956)
[5] Ragan, Bloxham, Specific labelling of a constituent polypeptide of bovine heart mitochondrial reduced nicotinamide-adenine dinucleotide-ubiquinone reductase by the inhibitor diphenylene iodonium, Biochem. J., 163, pp. 605-615, (1977)
[6] Leijh, Van Zmet, Ter Kuile, Van Furth, Effect of thioglycollate on phagocytic and microbiocidal activities of peritoneal macrophages, Infect. Immun., 46, pp. 448-452, (1984)
[7] Tsunawaki, Nathan, Enzymatic basis of macrophage activation: kinetic analysis of superoxide production in lystates of resident and activated mouse peritoneal macrophages and granulocytes, J. Biol. Chem., 259, pp. 4305-4312, (1984)
[8] Cross, Higson, Jones, Harper, Segal, The enzymic reduction and kinetics of oxidation of cytochrome b<sub>−245</sub> of neutrophils, Biochem. J., 204, pp. 479-485, (1982)
[9] Cross, Parkinson, Jones, The superoxide generating oxidase of leucocytes, Biochem. J., 223, pp. 337-344, (1984)
[10] Martin, Martin, Jeanin, Lagneau, Rat macrophage mediated toxicity to cancer cells: effect of endotoxins and endotoxin inhibitors contained in culture media, Eur. J. Immunol., 8, pp. 607-611, (1978)

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