USE OF N-15 DETERMINATIONS OF PURINE NITROGEN FRACTION OF DIGESTA TO DEFINE NITROGEN-METABOLISM TRAITS IN THE RUMEN

被引:47
作者
AHARONI, Y
TAGARI, H
机构
[1] Department of Animal Science, Faculty of Agriculture, Rehovot
关键词
PURINE NITROGEN; N-15; RUMEN;
D O I
10.3168/jds.S0022-0302(91)78431-2
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
A method for direct purine N isolation and determination was modified from the method of Zinn and Owens for rapid determination of purines. By this method, N derived from purines in digesta was measured and collected for N-15 determinations. Measurements of purine N in 33 samples of ruminal contents and duodenal digesta of sheep and goats were compared with purine determinations in the same samples. The results showed that essentially all the N in the purines that could be isolated by the Zinn and Owens procedure also could be detected by N isolation and determination. Determinations of N-15 in the NH3 N, NAN, and purine N fractions in a continuous culture in vitro showed significant differences in the N-15 enrichment curves between diets based on either roasted or raw soybean meal.
引用
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页码:2540 / 2547
页数:8
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