RICE MEMBRANES CONTAIN A CALCIUM-DEPENDENT PROTEIN-KINASE ACTIVITY WITH BIOCHEMICAL FEATURES OF ANIMAL PROTEIN-KINASE-C

被引：24

作者：

MORELLO, L ^{[1
]}

GIANI, S ^{[1
]}

CORAGGIO, I ^{[1
]}

BREVIARIO, D ^{[1
]}

机构：

[1] CNR, IST BIOSINTESI VEGETALI, VIA BASSINI 15, I-20133 MILAN, ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 197卷 / 01期

关键词：

D O I：

10.1006/bbrc.1993.2440

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence of calcium-dependent protein kinase activities in rice was investigated. Membrane preparations could phosphorylate the MARCKS peptide, a highly specific substrate for animal protein kinase C(PKC). Phosphorylation, strictly dependent on calcium, was specifically antagonized by a peptide whose amino acid sequence corresponds to the inhibitory, pseudosubstrate domain of mammalian PKC. Similar results have been obtained with rice soluble fractions. Addition of inhibitors of mammalian PKC (staurosporine and calphostin C) also inhibited phosphorylation of specific peptide substrates. Western blot analysis with anti-PKC antibodies identified three major bands (90, 87 and 54 kD) in rice membrane-associated proteins. © 1993 Acdamic Press. Inc.

引用

页码：55 / 61

页数：7

共 14 条

[1] CALCIUM-ACTIVATED PROTEIN-KINASE FROM SOLUBLE AND MEMBRANE-FRACTIONS OF MAIZE COLEOPTILES [J].

BATTEY, NH .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 170 (01) :17-22

[2] THE DIRECT MEASUREMENT OF PROTEIN-KINASE-C (PKC) ACTIVITY IN ISOLATED MEMBRANES USING A SELECTIVE PEPTIDE SUBSTRATE [J].

CHAKRAVARTHY, BR ;

BUSSEY, A ;

WHITFIELD, JF ;

SIKORSKA, M ;

WILLIAMS, RE ;

DURKIN, JP .

ANALYTICAL BIOCHEMISTRY, 1991, 196 (01) :144-150

[3] CROSS-REACTION OF A PLANT PROTEIN-KINASE WITH ANTISERUM RAISED AGAINST A SEQUENCE FROM BOVINE BRAIN PROTEIN-KINASE-C REGULATORY SUBUNIT [J].

ELLIOTT, DC ;

KOKKE, YS .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 145 (03) :1043-1047

[4]

FERRELL JE, 1991, METHOD ENZYMOL, V200, P430

[5] A CALCIUM-DEPENDENT BUT CALMODULIN-INDEPENDENT PROTEIN-KINASE FROM SOYBEAN [J].

HARMON, AC ;

PUTNAMEVANS, C ;

CORMIER, MJ .

PLANT PHYSIOLOGY, 1987, 83 (04) :830-837

[6] CALCIUM AND LIPID REGULATION OF AN ARABIDOPSIS PROTEIN-KINASE EXPRESSED IN ESCHERICHIA-COLI [J].

HARPER, JF ;

BINDER, BM ;

SUSSMAN, MR .

BIOCHEMISTRY, 1993, 32 (13) :3282-3290

[7] A CALCIUM-DEPENDENT PROTEIN-KINASE WITH A REGULATORY DOMAIN SIMILAR TO CALMODULIN [J].

HARPER, JF ;

SUSSMAN, MR ;

SCHALLER, GE ;

PUTNAMEVANS, C ;

CHARBONNEAU, H ;

HARMON, AC .

SCIENCE, 1991, 252 (5008) :951-954

[8] PROTEIN-KINASE-C CONTAINS A PSEUDOSUBSTRATE PROTOTYPE IN ITS REGULATORY DOMAIN [J].

HOUSE, C ;

KEMP, BE .

SCIENCE, 1987, 238 (4834) :1726-1728

[9] BIOCHEMICAL SIMILARITIES BETWEEN SOLUBLE AND MEMBRANE-BOUND CALCIUM-DEPENDENT PROTEIN-KINASES OF BARLEY [J].

KLIMCZAK, LJ ;

HIND, G .

PLANT PHYSIOLOGY, 1990, 92 (04) :919-923

[10] LOCALIZATION, SOLUBILIZATION AND CHARACTERIZATION OF PLANT MEMBRANE-ASSOCIATED CALCIUM-DEPENDENT PROTEIN-KINASES [J].

KLUCIS, E ;

POLYA, GM .

PLANT PHYSIOLOGY, 1988, 88 (01) :164-171

← 1 2 →