EPSTEIN-BARR-VIRUS DNA IN CEREBROSPINAL-FLUID FROM PATIENTS WITH AIDS-RELATED PRIMARY LYMPHOMA OF THE CENTRAL-NERVOUS-SYSTEM

被引：256

作者：

CINQUE, P

BRYTTING, M

VAGO, L

CASTAGNA, A

PARRAVICINI, C

ZANCHETTA, N

MONFORTE, AD

WAHREN, B

LAZZARIN, A

LINDE, A

机构：

[1] UNIV MILAN,S RAFFAELE HOSP,DEPT INFECT DIS,I-20122 MILAN,ITALY

[2] NATL BACTERIOL LAB,DEPT VIROL,S-10521 STOCKHOLM,SWEDEN

[3] KAROLINSKA INST,S-10401 STOCKHOLM 60,SWEDEN

[4] UNIV MILAN,L SACCO HOSP,DEPT PATHOL 5,I-20122 MILAN,ITALY

[5] UNIV MILAN,L SACCO HOSP,CENT ANAL LAB,I-20122 MILAN,ITALY

[6] UNIV MILAN,L SACCO HOSP,INFECT DIS CLIN,I-20122 MILAN,ITALY

来源：

LANCET | 1993年 / 342卷 / 8868期

关键词：

D O I：

10.1016/0140-6736(93)92814-A

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Epstein-Barr virus (EBV) is constantly associated with AIDS-related primary lymphomas of the central nervous system (CNS). To assess whether EBV DNA in cerebrospinal fluid (CSF) could be used as a tumour marker, CSF samples that had been taken within 180 days before death from 85 patients with HIV infection and neurological disorders at necropsy were examined retrospectively by nested polymerase chain reaction (PCR) for EBV. Histologically evident primary CNS lymphomas were found in 17 patients, and EBV was shown in tissue by in-situ hybridisation in 16 of the 16 cases examined. All 17 patients with primary CNS lymphoma had EBV DNA in CSF. EBV DNA was found in CSF from 1 of 68 HIV-infected patients without histologically detectable lymphoma at necropsy. PCR for EBV DNA in CSF was 100% sensitive and 98.5% specific for AIDS-associated primary CNS lymphoma, and may be useful as a diagnostic tumour marker.

引用

页码：398 / 401

页数：4

共 17 条

[1]

[Anonymous], 1982, CANCER, V49, P2112

[2] RAPID DIAGNOSIS OF HERPES-SIMPLEX ENCEPHALITIS BY NESTED POLYMERASE CHAIN-REACTION ASSAY OF CEREBROSPINAL-FLUID [J].