LIPOPOLYSACCHARIDE TREATMENT IN-VIVO INDUCES WIDESPREAD TISSUE EXPRESSION OF INDUCIBLE NITRIC-OXIDE SYNTHASE MESSENGER-RNA

被引：246

作者：

LIU, SF

ADCOCK, IM

OLD, RW

BARNES, PJ

EVANS, TW

机构：

[1] NATL HEART & LUNG INST, DEPT THORAC MED, LONDON SW3, ENGLAND

[2] UNIV WARWICK, DEPT BIOL SCI, COVENTRY CV4 7AL, W MIDLANDS, ENGLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 196卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.2380

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nitric oxide (NO) may mediate the hypotension of septic shock, but the effect of endotoxin on inducible NO synthase (iNOS) mRNA expression remains unclear. We studied the effects of lipopolysaccharide (LPS) treatment in vivo on iNOS mRNA expression using reverse transcription and polymerase chain reaction. The iNOS mRNA was absent or negligible in any tissue studied from control rats, but was markedly increased in lung, liver, spleen, skeletal muscle and kidney from LPS-treated rats. The LPS-induced increase in iNOS mRNA was prevented by dexamethasone. Our results indicate that LPS treatment in vivo induces the expression of an iNOS mRNA via a dexamethasone-sensitive mechanism, and thus provide direct molecular evidence for the involvement of NO in septic shock. © 1993 Academic Press, Inc.

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页码：1208 / 1213

页数：6

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