INTERACTION OF GUANINE-NUCLEOTIDES WITH [H-3] KAINATE AND 6-[H-3]CYANO-7-NITROQUINOXALINE-2,3-DIONE BINDING IN GOLDFISH BRAIN

Recent reports have suggested that a major proportion of [H-3]kainate binding in goldfish brain is to a novel form of G-protein-linked glutamate receptor. Here we confirm that guanine nucleotides decrease [H-3]kainate binding in goldfish brain membranes, but that binding is also reduced to a similar extent under conditions where G-protein modulation should be minimised. Inclusion of GTPgammaS resulted in an approximately twofold decrease in the affinity of [H-3]kainate binding and a 50% reduction in the apparent B(max) values in both Mg2+/Na+ and Mg2+/Na+-free buffer when assayed at 0-degrees-C. The pharmacology of [H-3]kainate binding is similar to that of well-characterised ionotropic kainate receptors but unlike that of known metabotropic glutamate receptors, with neither 1S,3R-amino-1,3-cyclopentanedicarboxylic acid (1S,3R-ACPD) nor ibotenic acid being effective competitors. The molecular mass of the [H-3]kainate binding protein, as determined by radiation inactivation, was 40 kDa, similar to the subunit sizes of other lower vertebrate kainate binding proteins that are believed to comprise ligand-gated ion channels. Furthermore, GTPgammaS also inhibited the binding of the non-NMDA receptor-selective antagonist 6-[H-3]cyano-7-nitroquinoxaline-2,3-dione. These data strongly suggest that the regulatory interaction between guanine nucleotides and [H-3]kainate and 6-[H-3]cyano-7-nitroquinoxaline-2,3-dione binding is complex and involves competition at the agonist/antagonist binding site in addition to any G-protein-mediated modulation.