2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS WITH IMMOBILIZED PH GRADIENTS IN THE FIRST DIMENSION (IPG-DALT) - THE STATE-OF-THE-ART AND THE CONTROVERSY OF VERTICAL VERSUS HORIZONTAL SYSTEMS

被引：201

作者：

GORG, A ^{[1
]}

BOGUTH, G ^{[1
]}

OBERMAIER, C ^{[1
]}

POSCH, A ^{[1
]}

WEISS, W ^{[1
]}

机构：

[1] TECH UNIV MUNICH,INST FOOD TECHNOL,W-8050 FREISING,GERMANY

来源：

ELECTROPHORESIS | 1995年 / 16卷 / 07期

关键词：

2-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS; IMMOBILIZED PH GRADIENTS;

D O I：

10.1002/elps.11501601183

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

After having established the basic protocol of two-dimensional electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt) in 1988 (A. Gorg et al., Electrophoresis 1988, 9, 531-546), some critical parameters of the actual IPG-Dalt protocols as well as the results obtained with horizontal and vertical second-dimensional sodium dodecyl sulfate-electrophoresis are demonstrated and discussed.

引用

页码：1079 / 1086

页数：8

共 29 条

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