ESTERASE STAINING OF HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS

被引：19

作者：

DEJONG, MCJM

机构：

[1] Department of Dermatology, State University of Groningen, Groningen

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 26卷 / 02期

关键词：

D O I：

10.1016/0022-1759(79)90082-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently proposed esterase staining methods for the cytochemical identification of human peripheral blood monocytes and lymphocytes in our hands gave suboptimal results. Cellular purification and recommended fixation procedures appeared to decrease the esterase reactivity of leucocyte preparations. Drying for 12-18 h without further fixation of cytocentrifuge smears was found to produce minimal loss of the staining capacity for 1-naphthyl butyrate esterase in mononuclear cells. It is hoped that the slightly modified procedure meets the need for a simple technique to define mononuclear blood cells for clinical purposes. © 1979.

引用

页码：193 / 196

页数：4