MICROINJECTION OF ANTIBODIES AGAINST SUPEROXIDE-DISMUTASE AND GLUTATHIONE-PEROXIDASE

被引:25
作者
MICHIELS, C
RAES, M
ZACHARY, MD
DELAIVE, E
REMACLE, J
机构
关键词
D O I
10.1016/0014-4827(88)90296-0
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Antibodies were prepared against glutathione peroxidase, superoxide dismutase, and catalase. Inhibition of the enzyme activity was obtained with anti-Gpx and anti-SOD antibodies but not with anti-CAT antibodies. The antibodies were then injected into human fibroblasts and bovine chondrocytes in culture either under normal conditions or under 1 atm of oxygen. The injected anti-Gpx and anti-SOD antibodies increased the mortality rate of the fibroblasts incubated under 1 atm of oxygen. However, when cells were incubated under normal atmosphere, anti-Gpx antibodies inhibited the division while anti-SOD antibodies increased this capacity. Anti-Gpx antibodies injected into chondrocytes decreased their viability. Injection of control antiserum had no effect. These data stress the primary importance of Gpx as antioxidant under all conditions and the relative efficiency of SOD according to the balance between the radical production and the activity of the other antioxidant systems.
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页码:581 / 589
页数:9
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共 48 条
[1]  
ANDREOLI SP, 1986, J LAB CLIN MED, V108, P190
[2]   IMPROVED SYSTEM FOR CAPILLARY MICRO-INJECTION INTO LIVING CELLS [J].
ANSORGE, W .
EXPERIMENTAL CELL RESEARCH, 1982, 140 (01) :31-37
[3]   DIRECT VISUALIZATION OF PROTEIN-TRANSPORT AND PROCESSING IN THE LIVING CELL BY MICROINJECTION OF SPECIFIC ANTIBODIES [J].
ARNHEITER, H ;
DUBOISDALCQ, M ;
LAZZARINI, RA .
CELL, 1984, 39 (01) :99-109
[4]   CHEMICAL COUPLING OF PEPTIDES AND PROTEINS TO POLYSACCHARIDES BY MEANS OF CYANOGEN HALIDES [J].
AXEN, R ;
PORATH, J ;
ERNBACK, S .
NATURE, 1967, 214 (5095) :1302-&
[5]   TISSUE FRACTIONATION STUDIES .17. INTRACELLULAR DISTRIBUTION OF MONOAMINE OXIDASE ASPARTATE AMINOTRANSFERASE ALANINE AMINOTRANSFERASE D-AMINO ACID OXIDASE + CATALASE IN RAT-LIVER TISSUE [J].
BAUDHUIN, P ;
BEAUFAY, H ;
SELLINGER, OZ ;
RAHMANLI, Y ;
DEDUVE, C ;
WATTIAUX, R ;
JACQUES, P .
BIOCHEMICAL JOURNAL, 1964, 92 (01) :179-+
[6]   INVOLVEMENT OF NUCLEAR LAMINS IN POSTMITOTIC REORGANIZATION OF CHROMATIN AS DEMONSTRATED BY MICROINJECTION OF LAMIN ANTIBODIES [J].
BENAVENTE, R ;
KROHNE, G .
JOURNAL OF CELL BIOLOGY, 1986, 103 (05) :1847-1854
[7]   INDEPENDENT REGULATION OF COLLAGEN TYPES BY CHONDROCYTES DURING THE LOSS OF DIFFERENTIATED FUNCTION IN CULTURE [J].
BENYA, PD ;
PADILLA, SR ;
NIMNI, ME .
CELL, 1978, 15 (04) :1313-1321
[8]   10-NM FILAMENTS ARE INDUCED TO COLLAPSE IN LIVING CELLS MICROINJECTED WITH MONOCLONAL AND POLYCLONAL ANTIBODIES AGAINST TUBULIN [J].
BLOSE, SH ;
MELTZER, DI ;
FERAMISCO, JR .
JOURNAL OF CELL BIOLOGY, 1984, 98 (03) :847-858
[9]   CYTOTOXICITY, DNA FRAGMENTATION AND SISTER-CHROMATID EXCHANGE IN CHINESE-HAMSTER OVARY CELLS EXPOSED TO THE LIPID-PEROXIDATION PRODUCT 4-HYDROXYNONENAL AND HOMOLOGOUS ALDEHYDES [J].
BRAMBILLA, G ;
SCIABA, L ;
FAGGIN, P ;
MAURA, A ;
MARINARI, UM ;
FERRO, M ;
ESTERBAUER, H .
MUTATION RESEARCH, 1986, 171 (2-3) :169-176
[10]   MICROINJECTION OF MESSENGER-RNA CODING FOR AN ANTI-GOLGI ANTIBODY INHIBITS INTRACELLULAR-TRANSPORT OF A VIRAL MEMBRANE-PROTEIN [J].
BURKE, B ;
WARREN, G .
CELL, 1984, 36 (04) :847-856