MATURATION OR DIFFERENTIATION OF HUMAN THYMOCYTE PRECURSORS INVITRO

被引:42
作者
GROH, V
FABBI, M
STROMINGER, JL
机构
关键词
D O I
10.1073/pnas.87.15.5973
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The differentiation or maturation potential of human thymocyte precursors has been studied by using a population of CD3/TCR-, CD4-, CD8- ('triple negative') thymocytes isolated by negative selection (TCR, T-cell receptor). This cell population, however, also contained 30-50% previously undescribed cells expressing very low levels of CD3/TCRγδ (CD3/TCRγδ(low); ~60% of which expressed the variable region gene Vδ1). Correspondingly, TCR γ and TCR δ gene rearrangements (predominantly Vδ1/joining region Jδ1) and full-length TCR γ and TCR δ transcripts (but only immature TCR β and no TCR α mRNAs) were found. These cells mobilized Ca2+ in response to ligation of CD3 but not following ligation of TCRγδ. When cultured in the presence of interleukin 7 or interleukin 2, these thymocytes gave rise to 30-60% CD3/TCRγδ(medium and high) cells (60-70% expressing Vδ1) seen as discrete populations. Thus, the proportion and Vδ phenotype of in vitro generated CD3/TCRγδ cells closely resembled those of CD3/TCRγδ(low) cells in freshly isolated 'thymocyte precursor' preparations. Small numbers of TCRαβ+ cells also appeared. It is thus uncertain whether maturation, differentiation, or both account for the appearance of mature CD3/TCR+ thymocytes, although the former appears most likely.
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页码:5973 / 5977
页数:5
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