A METHOD FOR RAPID FIXATION AND DEHYDRATION OF NEMATODE TISSUE FOR TRANSMISSION ELECTRON-MICROSCOPY

被引：19

作者：

JONES, JT ^{[1
]}

GWYNN, IA ^{[1
]}

机构：

[1] AFRC,IACR,ROTHAMSTED EXPTL STN,DEPT ENTOMOL & NEMATOL,HARPENDEN,HERTS,ENGLAND

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1991年 / 164卷

关键词：

TRANSMISSION ELECTRON MICROSCOPY; MICROWAVE OVEN; ACIDIFIED 2,2-DIMETHOXYPROPANE; NEMATODE; GLOBODERA-ROSTOCHIENSIS; CUTICLE; AMPHID;

D O I：

10.1111/j.1365-2818.1991.tb03190.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

A method is described using a microwave oven and acidified 2,2-dimethoxypropane to fix and dehydrate nematode tissue rapidly in preparation for transmission electron microscopy. Nematode tissue prepared using this method is well preserved compared with tissue fixed by conventional methods. The technique also enabled fixation of unhatched nematodes in the egg, a process that is almost impossible to achieve using conventional methods of fixation. Using the method described, differences were found between the cuticles of different nematode stages.

引用

页码：43 / 51

页数：9

共 16 条

[1]

Bird A. F., 1971, STRUCTURE NEMATODES

[2] SYNTHESIS OF CUTICULAR PROTEIN DURING THIRD MOLT IN NEMATODE NIPPOSTRONGYLUS-BRASILIENSIS [J].

BONNER, TP ;

WEINSTEIN, PP ;

SAZ, HJ .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY, 1971, 40 (1B) :121-+

[3]

Boon M. E., 1988, MICROWAVE COOKBOOK P

[4] A HOT ALDEHYDE-PEROXIDE FIXATION METHOD FOR ELECTRON-MICROSCOPY OF THE FREE-LIVING NEMATODE CAENORHABDITIS-ELEGANS [J].

BYARD, EH ;

SIGURDSON, WJ ;

WOODS, RA .

STAIN TECHNOLOGY, 1986, 61 (01) :33-38

[5] MOVEMENT OF GLOBODERA-ROSTOCHIENSIS (WOLLENWEBER) JUVENILES STIMULATED BY POTATO-ROOT EXUDATE [J].

CLARKE, AJ ;

HENNESSY, J .

NEMATOLOGICA, 1984, 30 (02) :206-212

[6]

CROFTON HD, 1971, PLANT PARASIT NEMAT, V1, P83

[7] INFLUENCE OF TEMPERATURE ON POLYPHENOL OXIDASE ACTIVITY IN 3 SPECIES OF HETERODERA [J].

ELLENBY, C ;

SMITH, L .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY, 1967, 21 (01) :51-&

[8] MICROWAVES FOR MICROSCOPY [J].

KOK, LP ;

BOON, ME .

JOURNAL OF MICROSCOPY, 1990, 158 :291-322

[9] CELLULAR ANATOMY OF EMBRYOS OF NEMATODE CAENORHABDITIS-ELEGANS - ANALYSIS AND RECONSTRUCTION OF SERIAL SECTION ELECTRON-MICROGRAPHS [J].

KRIEG, C ;

COLE, T ;

DEPPE, U ;

SCHIERENBERG, E ;

SCHMITT, D ;

YODER, B ;

VONEHRENSTEIN, G .

DEVELOPMENTAL BIOLOGY, 1978, 65 (01) :193-215

[10] RAPID CHEMICAL DEHYDRATION OF SAMPLES FOR ELECTRON-MICROSCOPIC EXAMINATIONS [J].

MULLER, LL ;

JACKS, TJ .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1975, 23 (02) :107-110

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