NATIVE COLLAGEN FIBRILS FROM ECHINODERMS ARE MOLECULARLY BIPOLAR

被引:40
作者
THURMOND, FA
TROTTER, JA
机构
[1] Department of Anatomy University, New Mexico School of Medicine Albuquerque
基金
美国国家科学基金会;
关键词
COLLAGEN; SYMMETRY; ANTIPARALLEL; FIBRIL; ECHINODERM;
D O I
10.1016/S0022-2836(05)80015-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Collagen fibrils are generally assumed to be cylinders with uniform diameters (except possibly at their ends) and to be composed of molecules all of which have the same polarity. These assumptions have been largely untested because of the extreme difficulty associated with isolating entire native fibrils. Intact collagen fibrils are readily extracted from certain echinoderms, however, and we have therefore analyzed the molecular structure of these fibrils. Our electron microscopic analyses show the above assumptions to be false: echinoderm fibrils, which previously have been shown to be symmetrically spindle shaped, are also molecularly bipolar. Their constituent molecules have their N-termini oriented toward the nearest fibril end, and they are antiparallel in the fibril center. The shape and molecular arrangement of these fibrils have implications for fibrillogenesis. © 1994 Academic Press Limited.
引用
收藏
页码:73 / 79
页数:7
相关论文
共 36 条
[1]   PROPOSED RESOLUTION OF THE PARADOX OF EXTENSIVE CROSSLINKING AND LOW TENSILE-STRENGTH OF CUVIERIAN TUBULE COLLAGEN FROM THE SEA-CUCUMBER HOLOTHURIA-FORSKALI [J].
BAILEY, AJ ;
GATHERCOLE, LJ ;
DLUGOSZ, J ;
KELLER, A ;
VOYLE, CA .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1982, 4 (06) :329-334
[2]  
BAILEY AJ, 1985, BIOL INVERTEBRATE LO, P369
[3]   SUPRAMOLECULAR STRUCTURE OF POLYMORPHIC COLLAGEN FIBRILS [J].
BRUNS, RR .
JOURNAL OF CELL BIOLOGY, 1976, 68 (03) :521-538
[4]  
Chapman J.A., 1985, BIOL INVERTEBRATE LO, V515, P537
[5]   STRUCTURE AND DEVELOPMENTAL EXPRESSION OF A SEA-URCHIN FIBRILLAR COLLAGEN GENE [J].
DALESSIO, M ;
RAMIREZ, F ;
SUZUKI, HR ;
SOLURSH, M ;
GAMBINO, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (23) :9303-9307
[6]  
DALESSIO M, 1990, J BIOL CHEM, V265, P7050
[7]  
DAVIS JS, 1988, ANNU REV BIOPHYS BIO, V17, P217
[8]  
DOYLE BB, 1974, PROC R SOC SER B-BIO, V187, P37, DOI 10.1098/rspb.1974.0059
[9]   COLLAGEN POLYMORPHISM - ITS ORIGINS IN AMINO-ACID SEQUENCE [J].
DOYLE, BB ;
HUKINS, DWL ;
HULMES, DJS ;
MILLER, A ;
WOODHEADGALLOWA.J .
JOURNAL OF MOLECULAR BIOLOGY, 1975, 91 (01) :79-&
[10]  
EXPOSITO JY, 1992, J BIOL CHEM, V267, P15559