A RENEWABLE GLUCOSE SENSOR FABRICATED FROM MICROEMULSION POLYMERIZATION OF THIOPHENE IN A FLOW CELL WITH APPLICATION IN A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SYSTEM

被引：13

作者：

CHAN, HSO

GAN, LM

CHI, H

TOH, CS

机构：

[1] Department of Chemistry, National University of Singapore, Kent Ridge

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1994年 / 379卷 / 1-2期

关键词：

GLUCOSE SENSOR; MICROEMULSION POLYMERIZATION; THIOPHENE; FLOW CELL; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY;

D O I：

10.1016/0022-0728(94)87150-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A glucose sensor was fabricated by the use of a microemulsion. The oil-in-water microemulsion was a simple three-component system consisting of a non-ionic surfactant, monomeric thiophene and aqueous buffer, without the use of an organic solvent. Enzyme was entrapped by the deposition of a thin layer of non-conducting polythiophene. This enzyme layer could be restored readily by the same microemulsion polymerization procedure when the enzyme was depleted. Application of this glucose sensor system in a flow-cell electrode was performed successfully. A linear range of over three orders of magnitude of glucose concentrations and a detection limit at the nanomole level were readily achieved. The capability of this sensor was demonstrated in connection with high-performance liquid chromatography for the direct sensing of glucose in fresh orange.

引用

页码：293 / 300

页数：8

共 36 条

[1] ELECTROCHEMICAL POLYMERIZATION OF POLY(THIOPHENE-3-ACETIC ACID), POLY(THIOPHENE-CO-THIOPHENE-3-ACETIC ACID) AND DETERMINATION OF THEIR MOLAR MASS [J].