INHIBITION OF DNASE ACTIVITY IN PFGE ANALYSIS OF DNA FROM CAMPYLOBACTER-JEJUNI

被引：97

作者：

GIBSON, JR ^{[1
]}

SUTHERLAND, K ^{[1
]}

OWEN, RJ ^{[1
]}

机构：

[1] CENT PUBL HLTH LAB,NATL COLLECT TYPE CULTURES,LONDON NW9 5HT,ENGLAND

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1994年 / 19卷 / 05期

关键词：

D O I：

10.1111/j.1472-765X.1994.tb00474.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

DNAse-positive strains of Campylobacter jejuni degrade their chromosomal DNA during standard preparative procedures before pulsed-field gel electrophoresis (PFGE). A simple method for inactivation of this DNAse activity is described. Formaldehyde fixation of the bacterial cells resulted in the preservation of the DNA in a state suitable for restriction digestion and subsequent electrophoretic analysis.

引用

页码：357 / 358

页数：2

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