ASSESSMENT OF LIPOXYGENASE ACTIVITY IN SEED EXTRACTS FROM 35 PLANT-SPECIES

被引：9

作者：

FAUCONNIER, ML

VANZEVEREN, E

MARLIER, M

LOGNAY, G

WATHELET, JP

SEVERIN, M

机构：

[1] U.E.R. Chimie Générale et Organique, Faculté des Sciences Agronomiques, 2, Passage des déportés, Gembloux

来源：

GRASAS Y ACEITES | 1995年 / 46卷 / 01期

关键词：

LIPOXYGENASE (ACTIVITY); SEED EXTRACT; VEGETABLE;

D O I：

10.3989/gya.1995.v46.i1.895

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Lipoxyganase activity in 35 seed extracts was determined an the basis of hydroperoxide synthesis using linoleic acid as substrate. The results referring to the extracted protein content show that several species of Vigna (V. unguiculata, V. radiata and V. mungo) and one of Trifolium exhibit stronger lipoxygenase activity than soybean enzyme extracts. The pH-activity relationship was also established for 4 very active samples. GCMS analysis revealed equal amounts of 9- and 13 hydroperoxides of linoleic acid In these seed extracts, indicating no enzyme positional specificity.

引用

页码：6 / 10

页数：5

共 15 条

[1]

Arctander S., Perfume and Flavor Chemicals, (1969)

[2]

Axelrod B., Cheesbrough T.M., Laakso S., Lipoxygenase from soybeans, Meth. Enzymol., 71, pp. 441-451, (1981)

[3]

Chism G.M., Soy lipoxygenase, Flavour Chemistry of Fats and Oils, (1985)

[4]

Croft K., Juttner F., Slusarenko A., Volatile products of the lipoxygenase pathway evolved from Phaseolus vulgaris (L) leaves inoculated with Pseudomonas syringae pv phaseolicola, Plant Physiol., 101, pp. 13-24, (1993)

[5]

Gardner H.W., Plattner R.D., Linoleate hydroperoxides are cleaved heterolytically into aldehydes by a Lewis acid in aprotic solvent, Lipids, 19, pp. 294-299, (1984)

[6]

Gardner H.W., How the lipoxygenase pathway affects the organoleptic properties of fresh fruit and vegetables, Flavor Chemistry of Lipid Foods, (1989)

[7]

Gardner H.W., Flavors and bitter tastes from oxidation of lipids by enzymes, Flavour Chemistry of Fats and Oils, (1985)

[8]

Gardner H.W., Recent investigations into the lipoxygenase pathway of plants, B. B. A., 1084, pp. 221-239, (1991)

[9]

Hamberg M., Samuelsson B., On the specificity of the oxygenation of unsaturated fatty acids catalized by soybean lipoxidase, J. Biol. Chem., 242, 22, pp. 5329-5335, (1967)

[10]

Hildebrand D.F., Kito M., Role of lipoxygenases in soybean seed protein quality, J. Agric. Food Chem., 32, pp. 815-819, (1984)

← 1 2 →