USE OF FLOW-CYTOMETRY IN ENOLOGY TO ANALYZE YEASTS

被引:24
作者
BRUETSCHY, A
LAURENT, M
JACQUET, R
机构
[1] COM INTERPROFESS VIN CHAMPAGNE,EPERNAY,FRANCE
[2] CHEMUNEX,MAISONS ALFORT,FRANCE
关键词
D O I
10.1111/j.1472-765X.1994.tb00885.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Flow cytometry is a rapid method with many microbiological applications. This technique can be used to obtain counts of viable yeasts in 30 min, whereas a 48 h incubation is necessary with plate counts. This rapid method was tested for its suitability to analyse wine yeasts in a multicentre study in our three laboratories. The study compares measurements obtained by flow cytometry and the usual method, in order to test the reliability of the new method. The results obtained were very similar in terms of both the micro-organisms detected and the precision of measurements.
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收藏
页码:343 / 345
页数:3
相关论文
共 13 条
[1]  
BOURGEOIS CM, 1991, TECHNIQUES ANAL CONT, P25
[2]  
BOYE E, 1983, J GEN MICROBIOL, V129, P973
[3]  
COOTES RL, 1980, FOOD TECHNOL AUST, V32, P522
[4]   EFFECTS OF IMMOBILIZATION ON GROWTH, FERMENTATION PROPERTIES, AND MACROMOLECULAR-COMPOSITION OF SACCHAROMYCES-CEREVISIAE ATTACHED TO GELATIN [J].
DORAN, PM ;
BAILEY, JE .
BIOTECHNOLOGY AND BIOENGINEERING, 1986, 28 (01) :73-87
[5]  
Dumain P. P., 1990, Biotech Forum Europe, V7, P224
[6]  
FROUDIERE I, 1988, Connaissance de la Vigne et du Vin, V22, P237
[7]  
GARDINI F, 1990, Italian Journal of Food Science, V2, P103
[8]  
Gillet R., 1981, Annales des Falsifications et de l'Expertise Chimique, V74, P185
[9]  
JONES RP, 1987, PROCESS BIOCHEM, V22, P118
[10]  
LONVAUD-FUNEL A, 1982, Connaissance de la Vigne et du Vin, V16, P241