OCTOPUS-PALLIDUS CYTOCHROME-P-450 - CHARACTERIZATION AND INDUCTION STUDIES WITH BETA-NAPHTHOFLAVONE AND AROCLOR-1254

被引：26

作者：

CHEAH, DMY ^{[1
]}

WRIGHT, PFA ^{[1
]}

HOLDWAY, DA ^{[1
]}

AHOKAS, JT ^{[1
]}

机构：

[1] RMIT UNIV,KEY CTR APPL & NUTR TOXICOL,MELBOURNE,VIC 3001,AUSTRALIA

来源：

AQUATIC TOXICOLOGY | 1995年 / 33卷 / 3-4期

关键词：

OCTOPUS PALLIDUS; CYTOCHROME P-450; EROD; ECOD; BETA-NAPHTHOFLAVONE; AROCLOR; 1254;

D O I：

10.1016/0166-445X(95)00014-U

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

Levels of cytochrome P-450 and related enzyme activities in Octopus pallidus were investigated for the first time to assess their potential for use as biomarkers of xenobiotic exposure. Spectral analysis revealed cytochrome P-450 in the digestive gland microsomes. Enzyme assays 7-ethoxyresorufin O-deethylase and 7-ethoxycoumarin O-deethylase were optimised with respect to temperature, pH and incubation time and NADPH cytochrome c reductase assays were performed. Corn oil was demonstrated to have an inhibitory effect on octopus cytochrome P-450 system. The study with Aroclor 1254 suspended in glycerol (100 mg/kg) revealed an elevation of 7-ethoxycoumarin O-deethylase activity, which would indicate cytochrome P-450 was induced.

引用

页码：201 / 214

页数：14

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