PHYSICO-CHEMICAL PROPERTIES OF SOME VACCINIAL IMMUNOPRECIPITINOGENS

Vaccinial immunoprecipitinogens, referred to as the e-f antigens were found to have some immunological and chemical properties attributed to the classical LS antigen of vaccinia virus. The e-f antigens were isolated and purified from vaccinia virus infected chorioallantoic membranes (CAM). The technique used for separation involved chromatography on DEAE-cllulose, gelfiltration on Sephadex G-200 and separation on hydroxylapatite. The amino acid composition of the antigens was compared with that of CAM and preparations of purified vaccinia virus. Sedimentation analysis indicated the relationship between the e-f antigens and a 6 S component with a molecular weight calculated to 96,600. Immunodiffusion studies suggested that the e-f antigens contained three immunoprecipitinogens, two of which were found to be thermo-resistant but sensitive to trypsin, while the third was a heat-labile antigen relatively more stable against trypsin digestion. © 1969 Springer-Verlag.