RESOLUTION AND RECONSTITUTION OF CYTOCHROME-OXIDASE

被引：5

作者：

FRY, M

机构：

[1] Institute for Enzyme Research University of Wisconsin-Madison, Madison

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 04期

关键词：

D O I：

10.1016/0006-291X(79)91151-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytochrome oxidase has been resolved into two fractions by extraction with formic acid. An insoluble-fraction (subunits I-III) retains the heme and phospholipid of the original enzyme and a soluble-fraction (subunits IV-VII) retains the copper. Recombination of the two enzymatically inactive fractions in the presence of sodium deoxycholate results in the expression of full enzymic activity. © 1979.

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页码：1119 / 1124

页数：6

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