TRANSVERSE DISTRIBUTION AND MOVEMENT OF LYSOPHOSPHATIDYLCHOLINE IN SARCOPLASMIC-RETICULUM MEMBRANES AS DETERMINED BY C-13-NMR AND LYSOPHOSPHOLIPASE

被引：21

作者：

VANDENBESSELAAR, AMHP ^{[1
]}

DEKRUIJFF, B ^{[1
]}

VANDENBOSCH, H ^{[1
]}

VANDEENEN, LLM ^{[1
]}

机构：

[1] STATE UNIV UTRECHT,DEPT BIOCHEM,UTRECHT,NETHERLANDS

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 555卷 / 02期

关键词：

(Sarcoplasmic reticulum membrane); !sup]13[!/sup]C-NMR; !sup]31[!/sup]P-NMR; Lysophosphatidylcholine; Lysophospholipase; Transbilayer movement;

D O I：

10.1016/0005-2736(79)90159-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. The transverse distribution of 1-palmitoyl-sn-glycero-3-phospho-N-[Me-13C]-choline in vitro incorporated in sarcoplasmic reticulum has been measured by means of 13C NMR and DyCl3 as an impermeable shift reagent. 2. 2. Lysophosphatidylcholine added to the membranes equilibrates within 30 min at 20°C between outer and inner membrane leaflet so that 42% is located in the inner leaflet. 3. 3. Lysophosphatidylcholine diffuses back from the inner leaflet to the outer upon lysophospholipase action on the outer lysophosphatidylcholine pool. © 1979.

引用

页码：193 / 199

页数：7

共 21 条

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