NEW GRADIENT FORMER AND A SIMPLIFIED PROCEDURE FOR ZONAL CENTRIFUGATION OF IMMUNE-COMPLEXES

被引：5

作者：

STEENSGAARD, J

JACOBSEN, C

机构：

[1] Institute of Medical Biochemistry, University of Aarhus

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 29卷 / 02期

关键词：

D O I：

10.1016/0022-1759(79)90067-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new gradient former for production of optimized sucrose gradients for B-XIV zonal rotors is described. Optimized gradients are gradients with an initial shelf to provide capacity and a shape that ensures isokinetic sedimentation conditions. By exhaustive computer calculations it has been found that optimized gradients can be produced by a very simple two-compartment gradient former. The design is given. Moreover, graphs for choice of gradient strength and run duration for preparative and analytical runs of samples containing soluble immune complexes are calculated. The new gradient former allows the use of a very simple procedure for zonal centrifugation. © 1979.

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页码：173 / 183

页数：11

相关论文

共 20 条

[1] ANALYTICAL TECHNIQUES FOR CELL FRACTIONS .7. A SIMPLE GRADIENT-FORMING APPARATUS [J].

ANDERSON, NG ;

RUTENBERG, E .

ANALYTICAL BIOCHEMISTRY, 1967, 21 (02) :259-+

[2] ANALYTICAL TECHNIQUES FOR CELL FRACTIONS .V. CHARACTERISTICS OF B-14 AND B-15 ZONAL CENTRIFUGE ROTORS [J].

ANDERSON, NG ;

WATERS, DA ;

FISHER, WD ;

CLINE, GB ;

NUNLEY, CE ;

ELROD, LH ;

RANKIN, CT .

ANALYTICAL BIOCHEMISTRY, 1967, 21 (02) :235-&

[3]

ANDERSON NORMAN G., 1966, NAT CANCER INST MONOGR, V21, P9

[4] MOLECULAR COMPOSITION AND SEDIMENTATION CHARACTERISTICS OF SOLUBLE ANTIGEN-ANTIBODY COMPLEXES [J].

AREND, WP ;

MANNIK, M ;

TELLER, DC .

BIOCHEMISTRY, 1972, 11 (22) :4063-&

[5] CIRCULATING IMMUNE-COMPLEXES IN PATIENTS WITH ATOPIC ALLERGY [J].

CANO, PO ;

CHOW, M ;

JERRY, LM ;

SLADOWSKI, JP .

CLINICAL ALLERGY, 1977, 7 (02) :167-171

[6] CIRCULATING IMMUNE-COMPLEXES IN SERA OF PATIENTS WITH BURKITTS-LYMPHOMA AND NASOPHARYNGEAL CARCINOMA [J].

HEIMER, R ;

KLEIN, G .

INTERNATIONAL JOURNAL OF CANCER, 1976, 18 (03) :310-316

[7]

JACOBSEN C, IMMUNOCHEMISTRY

[8] SIMPLE NUMERICAL-METHOD FOR CONSTRUCTION OF ISOKINETIC SUCROSE DENSITY GRADIENTS, AND THEIR APPLICATION TO CHARACTERIZATION OF IMMUNOGLOBULIN COMPLEXES [J].

JOHNS, P ;

STANWORTH, DR .

JOURNAL OF IMMUNOLOGICAL METHODS, 1976, 10 (2-3) :231-252

[9] STABILITY REGION OF ALBUMIN ZONES IN SUCROSE DENSITY GRADIENTS AT 1 G [J].

MEUWISSE.JA ;

HEIRWEGH, KP .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1970, 41 (03) :675-&

[10] CHARACTERIZATION OF MACROMOLECULES BY CONSTANT VELOCITY SEDIMENTATION [J].

NOLL, H .

NATURE, 1967, 215 (5099) :360-&