BIOLOGICAL CRYOSECTION PREPARATION AND PRACTICAL ION YIELD EVALUATION FOR ION MICROSCOPIC ANALYSIS

被引：18

作者：

SOD, EW ^{[1
]}

CROOKER, AR ^{[1
]}

MORRISON, GH ^{[1
]}

机构：

[1] CORNELL UNIV,DEPT CHEM,ITHACA,NY 14853

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1990年 / 160卷

关键词：

calcium; cryomicrotomy; Ion microscopy; microtomy; potassium; practical ion yield; rat liver; rat small intestine; SIMS; sodium;

D O I：

10.1111/j.1365-2818.1990.tb03047.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

A mounting method utilizing an indium substrate is described for preparing freeze‐dried cryosections of biological tissue for ion microscopic analysis. Using this procedure, a qualitative comparison between cryosection, conventional chemical, and freeze‐substitution specimen preparations is made with rat liver tissue. Practical ion yield variations in cryosections are found to be minimal (± 13% relative standard deviation) in tissue‐containing areas of rat liver and small intestine. 1990 Blackwell Science Ltd

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页码：55 / 65

页数：11

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