THE USE OF IMMOBILIZED CONCANAVALIN A FOR THE SEPARATION OF ALTERNANSUCRASE FROM DEXTRANSUCRASE IN CULTURE BROTH OF LEUCONOSTOC MESENTEROIDES NRRL B-1355

被引：6

作者：

Cote, Gregory L. ^{[1
]}

机构：

[1] Agr Res Serv, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA

来源：

BIOTECHNOLOGY TECHNIQUES | 1992年 / 6卷 / 01期

关键词：

D O I：

10.1007/BF02438686

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Immobilized concanavalin A has been used to bind a polysaccharide-glucosyltransferase complex from Leuconostoc mesenteroides NRRL B-1355, which is capable of synthesizing the unusual D-glucan alternan from sucrose. The dextransucrase present in culture fluid passes through a column of immobilized concanavalin A without binding, while the portion of alternansucrase that does bind is eluted using 1-O-methyl alpha-D-mannopyranoside.

引用

页码：45 / 48

页数：4

共 8 条

[1] ISOLATION AND PARTIAL CHARACTERIZATION OF AN EXTRACELLULAR GLUCANSUCRASE FROM LEUCONOSTOC-MESENTEROIDES NRRL B-1355 THAT SYNTHESIZES AN ALTERNATING (1-]6), (1-]3)-ALPHA-D-GLUCAN [J].

COTE, GL ;

ROBYT, JF .

CARBOHYDRATE RESEARCH, 1982, 101 (01) :57-74

[2] THE FORMATION OF ALPHA-D-(1-]3) BRANCH LINKAGES BY A D-GLUCANSUCRASE FROM STREPTOCOCCUS-MUTANS 6715 PRODUCING A SOLUBLE D-GLUCAN [J].

COTE, GL ;

ROBYT, JF .

CARBOHYDRATE RESEARCH, 1984, 127 (01) :95-107

[3] PROTEIN-CARBOHYDRATE INTERACTION .I. INTERACTION OF POLYSACCHARIDES WITH CONCANAVALIN A [J].

GOLDSTEIN, IJ ;

HOLLERMAN, CE ;

MERRICK, JM .

BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 97 (01) :68-+

[4]

HARDIN B, 1991, AGR RES, V39, P27

[5] ASSESSMENT OF FRACTIONATION OF CARBOHYDRATES ON CONCANAVALIN-A-SEPHAROSE 4B BY AFFINITY CHROMATOGRAPHY [J].

KENNEDY, JF ;

ROSEVEAR, A .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1973, (19) :2041-2046

[6] PREPARATION OF 2 INSOLUBLE FORMS OF PHYTOHEMAGGLUTININ, CONCANAVALIN-A, AND THEIR INTERACTIONS WITH POLYSACCHARIDES AND GLYCOPROTEINS [J].

LLOYD, KO .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1970, 137 (02) :460-&

[7]

LOPEZMUNGUIA A, 1990, ANN NY ACAD SCI, V614, P717

[8] STRUCTURE OF THE DEXTRAN OF LEUCONOSTOC-MESENTEROIDES-B-1355 [J].

MISAKI, A ;

TORII, M ;

SAWAI, T ;

GOLDSTEIN, IJ .

CARBOHYDRATE RESEARCH, 1980, 84 (02) :273-285

← 1 →