AGGREGATE OF AMPHIPHILIC BLOCK-COPOLYMER DERIVED FROM POLY[(N-ACYLIMINO)ETHYLENE]S AND GUEST-BINDING PROPERTIES FOR 8-ANILINO-1-NAPHTHALENESULFONIC ACID, PYRENE, AND ENZYMES

被引：30

作者：

NAKA, K

KUBO, Y

OHKI, A

MAEDA, S

机构：

[1] Department of Applied Chemistry and Chemical Engineering, Kagoshima University, Kagoshima

来源：

POLYMER JOURNAL | 1994年 / 26卷 / 03期

关键词：

POLYMER AGGREGATE; AMPHIPHILIC BLOCK COPOLYMER; POLY[(N-ACYLIMINO)ETHYLENE; GEL-FILTRATION; FLUORESCENT PROBE; ENZYME; LIPASE; POLYMER-ENZYME HYBRID; ORGANIC SOLVENT;

D O I：

10.1295/polymj.26.243

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The aggregate of an amphiphilic block copolymer consisting of poly[(N-acetylimino)ethylene] and poly[(N-pentanoylimino)ethylene] was prepared and its host properties for hydrophobic molecules in aqueous solution were studied by gel-filtration and a fluorescent probe. The aggregate showed strong guest-binding ability for 8-anilino-1-naphthalenesulfonic acid (ANS) and pyrene compared with its monomeric form. The interactions of three enzymes, horseradish peroxidase (HRP), Lipase OF, and Lipase P, to the aggregate were investigated by ultrafiltration and gel filtration analyses. The hydrolysis activities of Lipase OF and Lipase P in aqueous solution increased about 30% in the presence of the aggregate. The polymer-lipase P aggregate showed remarkably enhanced activity in comparison with the native enzyme in water-saturated organic solvent.

引用

页码：243 / 249

页数：7

共 13 条

[1]

AKIYOSHI K, 1992, CHEM LETT, P1227

[2]

BROCKMAN HL, 1973, J BIOL CHEM, V248, P4965

[3] MECHANISM OF PANCREATIC LIPASE ACTION .1. INTERFACIAL ACTIVATION OF PANCREATIC LIPASE [J].