DETERMINATION OF SULFATED DISACCHARIDES FROM CHONDROITIN SULFATES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：111

作者：

HJERPE, A ^{[1
]}

ANTONOPOULOS, CA ^{[1
]}

ENGFELDT, B ^{[1
]}

机构：

[1] UNIV PATRAS,ORGAN CHEM LAB,PATRAS,GREECE

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 171卷 / APR期

关键词：

D O I：

10.1016/S0021-9673(01)95313-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive method for the determination of chondroitin 4- and 6-sulphate is presented. After chondroitinase digestion of the chondroitin sulphate preparations, the obtained disaccharides are separated on a weak anion-exchange resin in a high-performance liquid chromatography system. The method was used to study 4-sulphate to 6-sulphate ratios in chondroitin sulphates prepared from bovine nasal cartilage and human nucleus pulposus. The results show clearly that these two preparations contain considerable amounts of both isomers. © 1979.

引用

页码：339 / 344

页数：6

共 12 条

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