MEMBRANE-TRANSPORT IN RESEALED HEMOGLOBIN-CONTAINING HUMAN-ERYTHROCYTE GHOSTS PREPARED BY A DIALYSIS PROCEDURE

Resealed haemoglobin-containing erythrocyte 'ghosts' have been proposed as in vivo carriers for enzyme replacement therapy. Transport of substrates and metabolites into and out of the 'ghost' has been suggested to be a limiting factor in such therapy. Studies of the transport of L-phenylalanine and of uric acid in normal human erythrocytes and prepared 'ghosts', in which the transport of sodium ions and D-glucose was intact, have shown that transport characteristics of 'ghosts' are identical to those of normal erythrocytes with transport not being a quantitatively limiting factor. © 1979.