DETERMINATION OF 9-[(2-PHOSPHONYLMETHOXY)ETHYL]ADENINE IN RAT URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

被引：21

作者：

RUSSELL, JW ^{[1
]}

MARRERO, D ^{[1
]}

WHITEROCK, VJ ^{[1
]}

KLUNK, LJ ^{[1
]}

STARRETT, JE ^{[1
]}

机构：

[1] BRISTOL MYERS SQUIBB CO,PHARMACEUT RES INST,DEPT ANTIINFECT CHEM,WALLINGFORD,CT 06492

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 572卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80498-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic (HPLC) method for the determination of 9-[(2-phosphonylmethoxy)ethyl]adenine (PMEA) in urine is described. The procedure includes treatment of the urine sample with chloroacetaldehyde to form the fluorescent 1,N6-ethenoadenosine derivative, which was analyzed by reversed-phase HPLC with fluorometric detection. Validation of the method showed good sensitivity, precision and reproducibility. The method is useful for the study of urinary excretion of PMEA in the rat.

引用

页码：321 / 326

页数：6

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