CONSTRUCTION OF A BIOLUMINESCENT MYCOBACTERIUM AND ITS USE FOR ASSAY OF ANTIMYCOBACTERIAL AGENTS

被引：46

作者：

ANDREW, PW

ROBERTS, IS

机构：

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1993年 / 31卷 / 09期

关键词：

D O I：

10.1128/JCM.31.9.2251-2254.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To show, as a model system, that mycobacteria can express heterologous luciferase genes and that bioluminescence can be a rapid method of measuring antimycobacterial activity, a bioluminescent form of Mycobacterium smegmatis was made by transformation with a Mycobacterium-Escherichia coli shuttle vector containing the luxAB genes from Vibrio harveyi. The antimycobacterial effects of antibiotics and biocides could be assayed in real time by using bioluminescent M. smegmatis.

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页码：2251 / 2254

页数：4

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