ISOLATION AND CHARACTERIZATION OF A FORSSMAN ANTIGEN-BINDING LECTIN FROM VELVET BEAN (MUCUNA-DERRINGIANA) SEEDS

被引：12

作者：

MO, HQ ^{[1
]}

GOLDSTEIN, IJ ^{[1
]}

机构：

[1] UNIV MICHIGAN, DEPT BIOL CHEM, ANN ARBOR, MI 48109 USA

来源：

GLYCOCONJUGATE JOURNAL | 1994年 / 11卷 / 05期

关键词：

MUCUNA (MUCUNA DERRINGIANA) LECTIN; VELVET BEAN LECTIN; HEMAGGLUTININ; FORSSMAN ANTIGEN BINDING LECTIN;

D O I：

10.1007/BF00731278

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A Forssman antigen (GalNAc alpha 1-3GalNAc beta 1-3Gal alpha 1-4Gal beta 1-4Glc beta 1-1Cer)-binding lectin has been purified from velvet bean (Mucuna derringiana) seeds by a combination of affinity chromatography and reversed phase HPLC. This lectin agglutinates both native and trypsin-treated sheep erythrocytes as well as trypsinized rabbit erythrocytes, but neither native rabbit nor human erythrocytes, irrespective of blood group type. SDS-PAGE and gel filtration chromatography reveal the lectin to be a homodimer consisting of two 54 kDa subunits linked by non-covalent bonds. The results obtained by quantitative precipitation, haemagglutination inhibition and TLC overlay assays indicate that the Mucuna lectin specifically recognizes Forssman antigen and Forssman disaccharide (GalNAc alpha 1-3GalNAc)-related structures.

引用

页码：424 / 431

页数：8

共 31 条

[1] IMMUNOCHEMICAL STUDIES ON THE COMBINING SITES OF FORSSMAN HAPTEN REACTIVE HEMAGGLUTININS FROM DOLICHOS-BIFLORUS, HELIX-POMATIA, AND WISTARIA-FLORIBUNDA [J].