ENDOTHELIN-1 STIMULATES HUMAN MONOCYTES IN-VITRO TO RELEASE TNF-ALPHA, IL-1-BETA AND IL-6

被引：63

作者：

HELSET, E ^{[1
]}

SILDNES, T ^{[1
]}

SELJELID, R ^{[1
]}

KONOPSKI, ZS ^{[1
]}

机构：

[1] UNIV TROMSO,INST MED BIOL,DEPT EXPTL PATHOL,N-9038 TROMSO,NORWAY

来源：

MEDIATORS OF INFLAMMATION | 1993年 / 2卷 / 06期

关键词：

CYTOKINES; ENDOTHELIN-1; IL-1-BETA; IL-6; INFLAMMATION; MACROPHAGES; MONOCYTES; TNF-ALPHA;

D O I：

10.1155/S0962935193000596

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

INCREASED plasma- and tissue levels of endothelin-1 (ET1) during inflammatory diseases, have suggested a role of ET-1 in the pathophysiotogy of inflammatory reactions. The authors have studied the effect of ET-1 on cytokine release from monocytes and monocyte-derived macrophages. ET-1 increased secretion of TNF-alpha, IL-1beta and IL-6 in a dose- and time-dependent manner. Optimal ET-1 concentration ranged from 0.01 to 1 nM. The maximal response was a 200 to 400% increase in cytokine release. A time-course study revealed that the pattern of cytokines induced by ET-1 was different in monocytes and macrophages, although an early increase in TNF-alpha was observed in both monocyte and macrophage supernatants. In conclusion, ET-1 stimulates monocytes and macrophages to release cytokines thereby demonstrating a potential role for ET-1 in regulation of inflammatory responses.

引用

页码：417 / 422

页数：6

共 27 条

[1] PRODUCTION OF HYBRIDOMA GROWTH-FACTOR BY HUMAN-MONOCYTES [J].

AARDEN, LA ;

DEGROOT, ER ;

SCHAAP, OL ;

LANSDORP, PM .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1987, 17 (10) :1411-1416

[2]

ADERKA D, 1989, J IMMUNOL, V143, P3517

[3]

BAILLAU A, 1991, EUR J CLIN INVEST, V21, P559

[4]

BOYUM A, 1968, SCAND J CLIN LAB INV, VS 21, P77

[5]

BURCHETT SK, 1988, J IMMUNOL, V140, P3473

[6] TUMOR-NECROSIS-FACTOR (CACHECTIN) IS AN ENDOGENOUS PYROGEN AND INDUCES PRODUCTION OF INTERLEUKIN-1 [J].

DINARELLO, CA ;

CANNON, JG ;

WOLFF, SM ;

BERNHEIM, HA ;

BEUTLER, B ;

CERAMI, A ;

FIGARI, IS ;

PALLADINO, MA ;

OCONNOR, JV .

JOURNAL OF EXPERIMENTAL MEDICINE, 1986, 163 (06) :1433-1450

[7]

EHRENREICH H, 1992, NEW BIOL, V4, P147

[8] ENDOTHELINS, PEPTIDES WITH POTENT VASOACTIVE PROPERTIES, ARE PRODUCED BY HUMAN MACROPHAGES [J].

EHRENREICH, H ;

ANDERSON, RW ;

FOX, CH ;

RIECKMANN, P ;

HOFFMAN, GS ;

TRAVIS, WD ;

COLIGAN, JE ;

KEHRL, JH ;

FAUCI, AS .

JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 172 (06) :1741-1748

[9]

ELIAS JA, 1985, J IMMUNOL, V135, P3198

[10] A HIGHLY SENSITIVE CELL-LINE, WEHI-164 CLONE 13, FOR MEASURING CYTOTOXIC FACTOR TUMOR-NECROSIS-FACTOR FROM HUMAN-MONOCYTES [J].

ESPEVIK, T ;

NISSENMEYER, J .

JOURNAL OF IMMUNOLOGICAL METHODS, 1986, 95 (01) :99-105

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