EXPRESSION OF FUNCTIONAL INFLUENZA-VIRUS-A PROTEINS AND TEMPLATE FROM CLONED CDNAS IN VACCINIA VIRUS-INFECTED CELLS

cDNAs containing the coding sequences of influenza type A virus polymerase proteins (PB1, PB2 and PA) and nucleoprotein (NP) have been expressed in mammalian cells by T7 polymerase provided by a recombinant vaccinia virus. The resulting proteins are able to form a complex that can copy a negative sense influenza-like RNA, transcribed from input DNA by the T7 polymerase, into a positive sense RNA that is translated into active chloramphenicol acetyltransferase (CAT). In this system there is no requirement for helper virus or purified viral core proteins, thus it will allow manipulation of all proteins as well as template for studies of replication in influenza virus. (C) 1994 Academic Press, Inc.