SPONTANEOUS DELETION MUTANTS OF THE LACTOCOCCUS-LACTIS TEMPERATE BACTERIOPHAGE-BK5-T AND LOCALIZATION OF THE BK5-T ATTP SITE

被引：29

作者：

BOYCE, JD

DAVIDSON, BE

HILLIER, AJ

机构：

[1] CSIRO,DIV FOOD SCI & TECHNOL,MELBOURNE LAB,HIGHETT,VIC 3190,AUSTRALIA

[2] UNIV MELBOURNE,RUSSELL GRIMWADE SCH BIOCHEM & MOLEC BIOL,PARKVILLE,VIC 3052,AUSTRALIA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 11期

关键词：

D O I：

10.1128/AEM.61.11.4105-4109.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Spontaneous deletion mutants of the temperate lactococcal bacteriophage BK5-T were obtained when the phage was grown vegetatively on the indicator strain Lactococcus lactis subsp. cremoris H2. One deletion mutant was unable to form stable lysogens, and analysis of this mutant led to the identification of the BK5-T attP site and the integrase gene (int). The core sequences of the BK5-T attP and host attB regions are conserved in a number of lactococcal phages and L. lactis strains.

引用

页码：4105 / 4109

页数：5

共 22 条

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