THE DETECTION OF ACUTE PARALYSIS VIRUS IN VARROA-JACOBSONI BY THE USE OF A SIMPLE INDIRECT ELISA

被引：65

作者：

ALLEN, MF

BALL, BV

WHITE, RF

ANTONIW, JF

机构：

[1] ROTHAMSTED EXPTL STN, DEPT PLANT PATHOL, HARPENDEN AL5 2JQ, HERTS, ENGLAND

[2] ROTHAMSTED EXPTL STN, DEPT BIOCHEM, HARPENDEN AL5 2JQ, HERTS, ENGLAND

来源：

JOURNAL OF APICULTURAL RESEARCH | 1986年 / 25卷 / 02期

关键词：

D O I：

10.1080/00218839.1986.11100700

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

A simple indirect enzyme-linked immunosorbent assay (ELISA) was used to detect and quantify acute paralysis virus (APV) in individual female Varroa jacobsoni collected from naturally infested honeybee (Apis mellifera) colonies in the Netherlands and Germany. The assay was very specific and sensitive, detecting the virus in concentrations as low as 3 ng/ml. Mites collected from both adult honeybees and sealed brood cells contained APV but virus concentrations varied widely between individuals. The possible role of V. jacobsoni as a vector of honeybee virus diseases is discussed.

引用

页码：100 / 105

页数：6

共 17 条

[1] A COMPARISON OF SEROLOGICAL TECHNIQUES FOR DETECTING AND IDENTIFYING HONEYBEE VIRUSES [J].