VASOACTIVE-INTESTINAL-PEPTIDE STIMULATION OF HUMAN RENAL ADENYLATE-CYCLASE INVITRO

被引：5

作者：

CHARLTON, BG ^{[1
]}

NEAL, DE ^{[1
]}

SIMMONS, NL ^{[1
]}

机构：

[1] FREEMAN RD HOSP, DEPT UROL, NEWCASTLE UPON TYNE NE7 7DN, TYNE & WEAR, ENGLAND

来源：

JOURNAL OF PHYSIOLOGY-LONDON | 1990年 / 423卷

基金：

英国惠康基金;

关键词：

D O I：

10.1113/jphysiol.1990.sp018034

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

1. A direct action of vasoactive intestinal peptide (VIP) upon human kidney was sought by measurement of renal adenylate cyclase in tissue homogenates and plasma membranes isolated from tissue samples excised for therapeutic reasons. 2. VIP (1 microM) produced a mean stimulation of adenylate cyclase activity of 3.5‐fold compared to basal values in cortical plasma membranes; comparative stimulations of 2.8‐fold and 27.3‐fold were obtained with 1 microM‐glucagon and 1 microM‐h(1‐34) parathyroid hormone respectively. 3. Half‐maximal stimulation of human renal cortical plasma membrane adenylate cyclase was observed with a mean value of 35 nM‐VIP. 4. The stimulation of renal adenylate cyclase by VIP appeared to be specific because stimulation by glucagon was additive to that obtained with VIP, and the VIP receptor antagonist (4 Cl‐D‐Phe6, Leu17)‐VIP inhibited the VIP‐dependent stimulation of adenylate cyclase activity. © 1990 The Physiological Society

引用

页码：475 / 484

页数：10

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