OCCURRENCE OF SELENOCYSTEINE IN THE SELENIUM-DEPENDENT FORMATE DEHYDROGENASE OF METHANOCOCCUS-VANNIELII

被引：84

作者：

JONES, JB ^{[1
]}

DILWORTH, GL ^{[1
]}

STADTMAN, TC ^{[1
]}

机构：

[1] NHLBI,BIOCHEM LAB,BETHESDA,MD 20014

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1979年 / 195卷 / 02期

关键词：

D O I：

10.1016/0003-9861(79)90351-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The selenium-dependent formate dehydrogenase of Methanococcus vannielii was isolated from bacteria grown in the presence of [75Se]selenite. Purification under strictly anaerobic conditions resulted in the simultaneous enrichment of formate dehydrogenase activity, 75Se, and a brown chromophore that absorbs maximally at 380 nm. Acid hydrolysis of the enzyme after reduction with borohydride and alkylation with iodoacetamide, released a radioactive selenoamino acid derivative that was identified as [75Se]carboxymethyl-selenocysteine. This is the third selenoenzyme shown to contain selenocysteine. © 1979.

引用

页码：255 / 260

页数：6

共 16 条

[1] FORMATE DEHYDROGENASE OF CLOSTRIDIUM-THERMOACETICUM - INCORPORATION OF SELENIUM-75, AND EFFECTS OF SELENITE, MOLYBDATE, AND TUNGSTATE ON ENZYME [J].