BIOCHEMICAL RESPONSE OF NORWAY SPRUCE (PICEA-ABIES (L) KARST) TOWARDS 14-MONTH EXPOSURE TO OZONE AND ACID MIST .2. EFFECTS ON PROTEIN-BIOSYNTHESIS

Four-year-old clonal Picea abies (L.) Karst. plants were treated with ozone (100 μm-3 plus peaks of 130 to 360 μm-3) and acid mist (pH 3·0) during two vegetation periods. Pulse labelling experiments on shoots were performed with [35S]methionine in the second year of exposure. Extraction of soluble needle proteins in citric acid buffer of pH 2·8 revealed protein patterns on SDS polyacrylamide gels that differed from those of control needles fumigated with ambient levels of ozone (50 μg m-3) and mist of pH 5·6. New proteins of MW 16000 and 32000 were synthesized only in ozone-exposed needles and could not be detected in the controls. © 1990.