CLONING AND EXPRESSION OF A COCAINE-SENSITIVE DOPAMINE TRANSPORTER COMPLEMENTARY-DNA

被引：539

作者：

SHIMADA, S

KITAYAMA, S

LIN, CL

PATEL, A

NANTHAKUMAR, E

GREGOR, P

KUHAR, M

UHL, G

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROL,BALTIMORE,MD 21224

[2] JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROSCI,BALTIMORE,MD 21224

来源：

SCIENCE | 1991年 / 254卷 / 5031期

关键词：

D O I：

10.1126/science.1948034

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A rat dopamine (DA) transporter complementary DNA has been isolated with combined complementary DNA homology and expression approaches. The DA transporter is a 619-amino acid protein with 12 hydrophobic putative membrane-spanning domains and homology to the norepinephrine and gamma-aminobutyric acid transporters. The expressed complementary DNA confers transport of [H-3]DA in Xenopus oocytes and in COS cells. Binding of the cocaine analog [H-3]CFT {[H-3]2-beta-carbomethoxy-3-beta-(4-fluorophenyl)tropane} to transfected COS cell membranes yields a pharmacological profile similar to that in striatal membranes.

引用

页码：576 / 578

页数：3

共 21 条

[1] EXPRESSION OF A HUMAN COCAINE-SENSITIVE DOPAMINE TRANSPORTER IN XENOPUS-LAEVIS OOCYTES [J].