INTERACTION BETWEEN ANTITUMOR ANTIBIOTIC CHROMOMYCIN A3 AND MG2+ .1. EVIDENCE FOR THE FORMATION OF 2 TYPES OF CHROMOMYCIN-A3-MG2+ COMPLEXES

被引：17

作者：

AICH, P ^{[1
]}

SEN, R ^{[1
]}

DASGUPTA, D ^{[1
]}

机构：

[1] SAHA INST NUCL PHYS, DIV BIOPHYS, 37 BELGACHIA RD, Kolkata 700037, INDIA

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 1992年 / 83卷 / 01期

关键词：

ANTITUMOR ANTIBIOTIC; CHROMOMYCIN-A3; MAGNESIUM ION; STOICHIOMETRY; CIRCULAR DICHROISM; FLUORESCENCE; KINETICS;

D O I：

10.1016/0009-2797(92)90089-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromomycin A3 (CHRA3) is an antitumor antibiotic which binds to Mg2+. In the present communication, we show, by means of equilibrium spectroscopic studies (such as absorption, fluorescence and circular dichroism), that two types of CHRA3-Mg2+ complexes (of 1:1 and 1.9:1 stoichiometries in terms of CHRA3:Mg2+, respectively) are formed depending on the concentrations of CHRA3 and Mg2+. The rate constant and activation energy for the formation of two complexes are different, thereby reinforcing the proposition that they are different molecular species. This observation is novel and significant in order to understand the anticancer property of the drug. It also provides explanation for earlier observations that site, affinity parameters and mode of interaction of the drug with DNA in the presence of Mg2+ depend on the relative concentration of Mg2+.

引用

页码：23 / 33

页数：11

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