DIFFERENTIAL EXPRESSION OF PDGF-A-CHAIN AND PDGF-B-CHAIN GENES AND PRODUCTION OF AA-DIMERS AND AB-DIMERS BY ACTIVATED HUMAN BLOOD MONOCYTES

被引：7

作者：

ALLAM, M

MARTINET, N

GALLATI, H

MARTINET, Y

机构：

[1] INSERM,U14,CO NO 10,F-54511 VANDOEUVRE NANCY,FRANCE

[2] CHU NANCY,PNEUMOL CLIN,F-54511 VANDOEUVRE NANCY,FRANCE

[3] F HOFFMANN LA ROCHE LTD,CVD,PHARMA RES,BASEL,SWITZERLAND

来源：

BIOCHIMIE | 1992年 / 74卷 / 12期

关键词：

PLATELET-DERIVED GROWTH FACTOR; BLOOD MONOCYTES; TISSUE FIBROSIS; TISSUE REPAIR;

D O I：

10.1016/0300-9084(92)90008-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Platelet-derived growth factor (PDGF) is composed of two chains (A and B) bound by disulfide bridges. Blood monocytes (BM) express the c-sis proto-oncogene, the gene coding for PDGF B-chain, and release PDGF. To evaluate PDGF release and A- and B-chain gene expression by BM, normal BM were cultured with LPS and specific transcripts for PDGF A- and B-chain genes were detected by Northern analysis and PDGF dimers by specific Elisas. Normal BM did not spontaneously express either A- or B-chain gene while, when activated, PDGF B-chain expression was precocious (maximum at 1 h) and decreased over 24 h; PDGF A-chain transcripts were present after 4 h and progressively increased over 24 h. Furthermore, activated BM released more AB dimers than AA dimers and almost no BB dimers. This observation confirms the concept of PDGF A- and B-chain separate gene regulation, and defines the specific molecular pattern of PDGF released by activated BM.

引用

页码：1097 / 1101

页数：5

共 37 条

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