CHARACTERIZATION OF SYMBIONIN WITH ANTISYMBIONIN ANTISERUM

被引：13

作者：

HARA, E ^{[1
]}

FUKATSU, T ^{[1
]}

ISHIKAWA, H ^{[1
]}

机构：

[1] UNIV TOKYO,FAC SCI,INST ZOOL,BUNKYO KU,TOKYO 113,JAPAN

来源：

INSECT BIOCHEMISTRY | 1990年 / 20卷 / 04期

关键词：

anti-symbionin antiserum; endosymbiosis; immuno-histochemistry; immunoblotting; pea aphid;

D O I：

10.1016/0020-1790(90)90064-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anti-symbionin antiserum was obtained by injecting symbionin purified from pea aphids into a rabbit. On immunoblotting, in addition to symbionin many smaller proteins cross-reacted with the antiserum suggesting that symbionin in the aphid tissue is prone to degradation. When estimated by immunoblotting with anti-symbionin antiserum, the symbionin content per unit wet weight of both symbiotic and aposymbiotic aphids was shown to decrease with age. Five distinct aphid species were shown to share a protein class related to symbionin in terms of molecular mass and immunogenicity. Immuno-histochemistry of aphid tissues with anti-symbionin antiserum indicated that symbionin is localized exclusively within the mycetocyte harboring the primary symbionts. © 1990.

引用

页码：429 / &

共 14 条

[1] Buchner P., 1965, ENDOSYMBIOSIS ANIMAL
[2] PURIFICATION AND PARTIAL CHARACTERIZATION OF SYMBIONIN, AN APHID ENDOSYMBIONT-SPECIFIC PROTEIN
HARA, E
ISHIKAWA, H
[J]. INSECT BIOCHEMISTRY, 1990, 20 (04): : 421 - 427
[3] HARA E, 1990, IN PRESS 4 P END LYO
[4] USE OF AVIDIN-BIOTIN-PEROXIDASE COMPLEX (ABC) IN IMMUNOPEROXIDASE TECHNIQUES - A COMPARISON BETWEEN ABC AND UNLABELED ANTIBODY (PAP) PROCEDURES
HSU, SM
RAINE, L
FANGER, H
[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1981, 29 (04) : 577 - 580
[5] INTRACELLULAR SYMBIONT AS A MAJOR SOURCE OF RIBOSOMAL-RNAS IN APHID MYCETOCYTES
ISHIKAWA, H
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1978, 81 (03) : 993 - 999
[6] SYMBIONIN, AN APHID ENDOSYMBIONT-SPECIFIC PROTEIN .1. PRODUCTION OF INSECTS DEFICIENT IN SYMBIONT
ISHIKAWA, H
YAMAJI, M
[J]. INSECT BIOCHEMISTRY, 1985, 15 (02): : 155 - +
[7] DNA, RNA AND PROTEIN-SYNTHESIS IN THE ISOLATED SYMBIONTS FROM THE PEA APHID, ACYRTHOSIPHON-PISUM
ISHIKAWA, H
[J]. INSECT BIOCHEMISTRY, 1982, 12 (06): : 605 - 612
[8] SYMBIONIN, AN APHID ENDOSYMBIONT-SPECIFIC PROTEIN .2. DIMINUTION OF SYMBIONIN DURING POST-EMBRYONIC DEVELOPMENT OF APOSYMBIOTIC INSECTS
ISHIKAWA, H
YAMAJI, M
HASHIMOTO, H
[J]. INSECT BIOCHEMISTRY, 1985, 15 (02): : 165 - +
[9] ALTERATION WITH AGE OF SYMBIOSIS OF GENE-EXPRESSION IN APHID ENDOSYMBIONTS
ISHIKAWA, H
[J]. BIOSYSTEMS, 1984, 17 (02) : 127 - 134
[10] AGE-DEPENDENT REGULATION OF PROTEIN-SYNTHESIS IN AN APHID ENDOSYMBIONT BY THE HOST INSECT
ISHIKAWA, H
[J]. INSECT BIOCHEMISTRY, 1984, 14 (04): : 427 - +

← 1 2 →