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SIALYLATED CARBOHYDRATE CHAINS OF RECOMBINANT HUMAN GLYCOPROTEINS EXPRESSED IN CHINESE-HAMSTER OVARY CELLS CONTAIN TRACES OF N-GLYCOLYLNEURAMINIC ACID

被引：100
作者：
HOKKE, CH
BERGWERFF, AA
VANDEDEM, GWK
VANOOSTRUM, J
KAMERLING, JP
VLIEGENTHART, JFG
机构：
[1] STATE UNIV UTRECHT,BIJVOET CTR,DEPT BIOORGAN CHEM,POB 80075,3508 TB UTRECHT,NETHERLANDS
[2] DIOSYNTH INT BV,OSS,NETHERLANDS
[3] CIBA GEIGY AG,DEPT BIOTECHNOL,CH-4002 BASEL,SWITZERLAND
来源：
FEBS LETTERS | 1990年 / 275卷 / 1-2期
关键词：
Chinese hamster ovary cell; N-Glycolylneuraminic acid; Recombinant human glycoprotein;
D O I：
10.1016/0014-5793(90)81427-P
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
HPLC analysis of sialic acid released from recombinant variants of human tissue plasminogen activator, human chimeric plasminogen activator, human erythropoietin, and human follitropin, expressed in Chinese hamster ovary cells, demonstrates for each glycoprotein the presence of N-acetylneuraminic and N-glycolylneuraminic acid in a ratio of 97:3. Structural analysis by 500 MHz1H-NMR spectroscopy, of the enzymatically released N-linked carbohydrate chains of chimeric plasminogen activator and of erythropoietin, showed that α2-3 linked N-glycolylneuraminic acid can occur in different N-acetyllactosamine type antennary structures. © 1990.
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页码：9 / 14
页数：6
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