SATURATION ANALYSIS IN RECEPTOR-BINDING ASSAYS - AN EVALUATION OF 6 DIFFERENT CALCULATION TECHNIQUES

Randomized errors to varying extents have been introduced in a constructed data set simulating receptor binding saturation analyses. The parameters B(max) and K(d) were then determined using six different evaluation techniques, four linear and two non-linear. It is concluded that a non-linear evaluation gives more reliable results, especially when errors are large. Furthermore, an inevitable correlation between B(max) and K(d) regardless of evaluation technique results in possible misinterpretations of simultaneous changes in both parameters.